Ekstraksi Polifenol Total dari Daun Kadamba (Mitragyna speciosa Korth) Menggunakan Metode Choline Chloride-Sorbitol based Microwave Assisted Extraction
DOI:
https://doi.org/10.30872/mpc.v10i.105Keywords:
Choline chloride-sorbitol, leaf Kadamba (Mitragyna speciosa Korth), Green solvent, microwave assisted extractionAbstract
This research was conducted to determine the influence of the use of the choline chloride-sorbitol based microwave assisted extraction (MAE) method against the total extraction of polyphenols from the leaf Kadamba (Mitragyna speciosa Korth). Simplisia extracted using solvent (green solvent) choline chloride-sorbitol with a ratio of 1:1; 1:2 and 1:3 g/g with sample and solvent ratio of 1:10 g/mL, microwave strength is 50% watt and performed for 10 minutes, the extract solution has been obtained and then done determination of total levels of polyphenols by using folin ciocalteu reagents and further measured its absorption with the UV-VIS spectrophotometer device at a wavelength of 770 nm. Based on the results of the study, obtained the levels of polyphenols from each comparison of solvent used against the sample of 334 mg of GAE/g samples (1:1 g/g solvent); 372 mg GAE/g samples (1:2 g/g solvents) and 299 mg of GAE/g samples (1:3 g/g solvents). Based on the results of the study can be concluded that the method of choline chloride-sorbitol based microwave assisted extraction (MAE) can be used to extract the polyphenols from Kadamba leaves easily, quickly and efficiently.
References
[1] Sivasothy, Y., Ahmad, S., Awang, K., Tohar, N., & Shilpi, J. A. 2016. Chemical constituents and nitric oxide inhibitory activity of supercritical carbon dioxide extracts from Mitragyna speciosa leaves. Arabian Journal of Chemistry.
[2] Ahmad, K., & Aziz, Z. 2012. Mitragyna speciosa use in the northern states of Malaysia: A cross-sectional study. Journal of Ethnopharmacology, 141(1), 446–450.
[3] Garg, C., Verma, S., Satija, S., Mehta, M., Dureja, H., & Garg, M. 2016. Microwave assisted extraction of bioactive compound phyllanthin from Phyllanthus amarus and optimization using central composite design. International Journal of Pharmaceutical Science and Research, 1(7), 30–35.
[4] Saqifah, N., Purbowati, E., & Rianto, E. 2014. Optimation Axtraction Using Microwave Assisted Extraction To Yield Green Tea Extract Optimasi Ekstraksi Ampas Teh Hijau (Camellia sinensis). Traditional Medicine Journal, 19(January), 29–35.
[5] C. Duarte, A. R., Paiva, A., Reis, R. L., Silva, J. M., Castro, V. I. B., & Craveiro, R. 2018. Natural deep eutectic systems as alternative nontoxic cryoprotective agents. Cryobiology, 83(June), 15–26.
[6] Savi, L. K., Dias, M. C. G. C., Carpine, D., Waszczynskyj, N., Ribani, R. H., & Haminiuk, C. W. I. 2018. Natural deep eutectic solvents (NADES) based on citric acid and sucrose as a potential green technology: a comprehensive study of water inclusion and its effect on thermal, physical and rheological properties. International Journal of Food Science and Technology, 1–10.
[7] Bobo-García, G., Davidov-Pardo, G., Arroqui, C., Vírseda, P., Marín-Arroyo, M. R., & Navarro, M. 2015. Intra-laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. Journal of the Science of Food and Agriculture, 95(1), 204–209.
[8] Wei, Z. F., Wang, X. Q., Peng, X., Wang, W., Zhao, C. J., Zu, Y. G., & Fu, Y. J. 2014. Fast and green extraction and separation of main bioactive flavonoids from Radix Scutellariae. Industrial Crops and Products, 63, 175–181.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Ramila Hidayati, Dewi Rahmawati, Islamudin Ahmad (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
