Ekstraksi Polifenol Total dari Herba Suruhan (Pepperomia pellucida (L)Kunth.) menggunakan Malic Acid-Glucose Based Microwave Assisted Extraction
DOI:
https://doi.org/10.30872/mpc.v12i.153Keywords:
glucose, green solvent, suruhan herbs (Pepperomia pellucida (L) Kunth), Malic acid, microwave extractionAbstract
This study aims to determine the effect of the use of malic acid-glucose based microwave assited extraction (MAE) methods on total polyphenol extraction from suruhan herbs (Pepperomia pellucida (L) Kunth.). Samples that have been dried in the extraction using solvents (green solvent) Malic Acid-Glucose with a ratio of 1: 2, 1:3 and 1: 4 g/g in several conditions including, microwave strength 30% for 5 minutes with a sample ratio and a solvent of 1:14 g/mL. Then the extract solution obtained was measured in total polyphenol levels using the folin ciocalteu (FC) reagent and the absorbance was measured at a wavelength of 658 nm using a UV-vis spectrophotometer. Based on the results of the study obtained levels of polyphenols from each condition of the solvent used is 85 mg GAE/g sample (1 : 2 g/g solvent), 696 mg GAE/ g sample (1:3 g / g solvent) and 317 mg GAE /g sample (1: 4 g / g solvent). From this study it can be concluded that the method of malic acid-glucose based microwave assited extraction can be used to extract polyphenol compounds from Suruhan herbs quickly, easily and efficiently.
References
Ahmad, I., Yanuar, A., Mulia, K., & Mun?im, A. (2017). Optimization of ionic liquid-based microwave-assisted extraction of polyphenolic content from Peperomia pellucida (L) Kunth using response surface methodology. Asian Pacific Journal of Tropical Biomedicine, 7(7), 660?665. https://doi.org/10.1016/j.apjtb.2017.06.010
Bobo-Garc?a, G., Davidov-Pardo, G., Arroqui, C., V?rseda, P., Mar?n-Arroyo, M. R., & Navarro, M. (2014). Intra-Laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. Journal of the Science of Food and Agriculture, 95, 204?209. https://doi.org/10.1002/jsfa.6706
Depkes RI. (1989). Parameter standar umum ekstrak tumbuhan obat, Jakarta: Departement Kesehatan Republik Indonesia. Edisi V, 9?11, 16. https://doi.org/615.32
Handayani,Dwi Handayani Mun?im, A., & Anna S. Ranti. (2014). Optimasi ekstraksi ampas teh hijau (Camellia sinensis) menggunakan metode microwave assisted extraction untuk menghasilkan ekstrak teh hijau. TraditionalMedicine Journal, 19, 29?35. https://doi.org/10.1080/10440040903303504
Hemalatha, S., Mandal, V., & Mohan, Y. (2007). Microwave assisted extraction - An innovative and promising extraction tool for 52.
Majumder, Pulak., Abraham, Priya., Satya V. 2011. Ethno-medicinal, Phytochemical and Pharmacological review of an amazing medicinal herb Peperomia pellucida (L.) HBK. Research Journal of Pharmaceutical, Biological and Chemical, Vol. 2, Issue 4,358-364
medicinal plant research. Pharmacognosy Reviews, 1(1), 7?18.
Karabegovi?, I. T., Stoji?evi?, S. S., Veli?kovi?, D. T., Nikoli?, N. ?., & Lazi?, M. L. (2013). Optimization of microwave-assisted extraction and characterization of phenolic compounds in cherry laurel (Prunus laurocerasus) leaves. Separation and Purification Technology, 120, 429?436. https://doi.org/10.1016/j.seppur.2013.10.021
Kurniasari, L., Hartati, I., & Ratnani, R. D. (2008). Kajian ekstraksi minyak jahe menggunakan Microwave Assisted Extraction (MAE) Jahe. Momentum, 4(1952), 47?52.
Wei, Z. F., Wang, X. Q., Peng, X., Wang, W., Zhao, C. J., Zu, Y. G., & Fu, Y. J. (2015). Fast and green extraction and separation of main bioactive flavonoids from Radix Scutellariae. Industrial Crops and Products, 63(1), 175?181. https://doi.org/10.1016/j.indcrop.2014.10.013
Yuntao, D., Jaapvan, S., Geert Jan, W., Robert, V., & Young Hae, C. (2013). Natural deep eutectic solvents as new potential media for green technology. Analytica Chimica Acta, 766(2013), 61?68. https://doi.org/10.1016/j.aca.2012.12.019
Zuo-fu, W., Xi-qing, W., Xiao, P., Wei, W., & Chun-jian, Z. (2014). Fast and green extraction and separation of main bioactive flavonoids from Radix Scutellariae. Industrial Crops & Products. https://doi.org/10.1016/j.indcrop.2014.10.013
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Copyright (c) 2020 Muhammad Safruddin, Nur Mita, Islamudin Ahmad (Author)
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