Zebrafish (Danio rerio) Sebagai Model Hewan Coba pada Pengujian Aktivitas Obat
DOI:
https://doi.org/10.30872/mpc.v11i.233Keywords:
Zebrafish, model hewan coba, aktivitas farmakologi, dataAbstract
Perkembangan metode penelitian menggunakan hewan coba sering terkendala pada aspek 3R (reduce, replace, refinement). Untuk melakukan skrining aktivitas peneliti sering dihadapkan pada pilihan menggunakan hewan coba golongan rodent dalam jumlah banyak. Sedangkan pilihan menggunakan larva udang menawarkan pilihan jenis pengujian yang terbatas. Penelitian ini bertujuan mengkaji keunggulan dan kelemahan metode uji aktivitas menggunakan model zebrafish (Danio rerio). Penelitian dilakukan secara metaanalisis menggunakan berbagai literatur yang diakses dari Pubmed, Sciencedirect, dan Google Scholar. Metode pengujian menggunakan zebrafish menunjukkan keunggulan pada pengamatan perilaku setelah pemberian bahan uji, terutama yang berkaitan dengan sistem saraf. Sedangkan untuk pengujian aktivitas terhadap jantung, pengamatan perilaku fisik kurang menunjukkan perbedaan yang berarti, namun penelusuran aktivitas terhadap otot jantung dapat dilakukan dengan metode pewarnaan histopatologi. Pengamatan molekular tersebut mampu menunjukkan kemampuan kontraksi dan relaksasi otot jantung. Pengamatan pada fungsi pengaturan tekanan darah dan fungsi sistem pencernaan juga dapat terpantau jelas jika menggunakan model zebrafish transgenik. Model zebrafish hasil rekayasa ini dapat terlihat bening sehingga ritme kerja organ dalamnya terrekam menghasilkan data pengamatan yang valid. Sehingga, untuk penelitian skrining uji aktivitas suatu bahan, penggunaan zebrafish menjadi salah satu pilihan yang tepat.
References
[1] Wang TY, Hsieh CH, Hung CC, Jao CL, Lin PY, Hsieh LH, Hsu KC. 2017. A study to evaluate the potential of an in silicoapproach for predicting dipeptidyl peptidase-IV inhibitory activity in vitroof protein hydrolysates, Food Chemistry, 234, (0),431-438. https://doi.org/10.1016/j.foodchem.2017.05.035
[2] Hsieh JH, Sedykh A, Mutlu E, Germolec DR, Auerbach SS, Rider CV. 2020. Harnessing in silico, in vitro, and in vivo data to understand the toxicity landscape of polycyclic aromatic compounds (PACs), Chem. Res. Toxicol., https://doi.org/10.1021/acs.chemrestox.0c00213
[3] Supraja N, Prasad TNVK, Gandhi AD, Anbumani D, Kavitha P, Babujanartharam R. 2018. Synthesis, characterization and evaluation of antimicrobial efficacy and brine shrimp lethality assay of Alstonia scholarisstem bark extract mediated ZnONPs, Biochemistry and Biophysics Reports, 14, (July 2018), 69-77. https://doi.org/10.1016/j.bbrep.2018.04.004
[4] Gandini S, Palli D, Spadola G, Bendinelli B, Cocorocchio E, Stanganelli I, et al.2018. Antihipertensive drugs and skin cancer risk: a review of the literature and meta-analysis, Critical Reviews in Oncology/Hematology, 122, (February 2018), 1-9. https://doi.org/10.1016/j.critrevonc.2017.12.003
[5] Akhter A, Kumangai R, Roy SR, Li S, Tokumoto M, Hossain B, Wang J, et al.2016. Generation of Transparent Zebrafish with Fluorescent Ovaries: A Living Visible Model for Reproductive Biology, Zebrafish,13, (3), 155-160. DOI: 10.1089/zeb.2015.1116
[6] Dee CT, Raghavendar T, Nagaraju, Emmanouil I, Gray C, Ama LF, Jhnston SA, et al. 2016. Cd4-Transgenic Zebrafish reveal tissue-resident Th2 and Regulatory T cell-like populations and Diverse Mononuclear Phagocytes, J. immunol, 197, (9), 3521-3530. https://doi.org/10.4049/jimmunol.1600959
[7] Sebille YZA, Gibson RJ,Wardhill HR. 2019. Use of Zebrafish to modelchemotherapy and targeted therapy gastrointestinal toxicity, Experimental Biology and Medicine https://doi.org/10.1177/1535370219855334
[8] Foster D, Ammer IA, Laurel E, Barker AJ, Fernandes AM. 2017. Genetic targeting and anatomic registration of neuronal populations in the zebrafish brain with new set of BAC transgenic tools, Scientific Reports, 7, (5230(2017)), 1-11. https://www.nature.com/articles/s41598-017-04657-x
[9] Leeuwen LM, Evans RJ, Jim KK, Verboom T,Fang X, Bojarczuc A, Malicki J, Johnston SA. 2018. A Transgenis zebrafish model for the in vivo study of the blood and choroid plexus brain barriers usiang claudin 5, Biology Open, 7, (bio030494), 1-12. doi:10.1242/bio.030494
[10] Manghnani PN, Wu W, Xu S, Hu F, The C, Liu B. 2018. Visualizing photodynamic therapy in transgenic zebrafish using organic nanoparticles with aggregation-induced emission, Nano-Micro Letters, 10, (61), 1-9. https://doi.org/10.1007/s40820-018-0214-4
[11] Clemson.edu. Daily care of zebrafish Danio rerio Facility: Aquatic animal research laboratory Room 3. Media. Clemson.edu
[12] Alestrom P, Angelo LD. 2020. Zebrafish: Housing and husbandry recommendations, Laboratory
Animals, 54, (3), 213-224.doi/full/10.1177/0023677219869037
[13] Kunst M, Laurell E, Mokayes N, Kramer A, Kuno F, Fernandes AM. Foster D, Maschio M, Baier H. 2019. A cellular-resolution atlas of the larval zebrafish brain, NeuroResource, 103, (1), 21-38.
[14] Chen X, Mu Y, Kuan AT, Nikitchenko M, Chen AB, Gavornik JP, Sompolinsky H, Engert F, Ahrens MB. 2018. Brain-wide organization of neuronal activity and convergent sensorimotor transformations in larval zebrafish, Neuron, 100, (4), 876-890.
[15] Beffagna G. 2019. Zebrafish as a smart modelto understand regeneration after heart injury: How fish could help humans, Front. Cardiovasc. Med.https://doi.org/10.3389/fcvm.2019.00107
[16] Annie NY, Curtain AJ, Mawdsley DJ, White S, Shin B. 2005. Formation of the digestive system in zebrafish:III. Intestinal epithelium morphogenesis, Developmental Biology, 286, (1),114-135. https://doi.org/10.1016/j.ydbio.2005.07.013
[17] Heilmann S, Ratnakumar K, Langdon EM, Kansler ER, Kin IS, et al., 2019. A quantitative system for studying metastasis using transparent zebrafish, Integrated systems and Technologies, 4272-4282. 10.1158/0008-5472.CAN-14-3319
[18] Ama NS, Jones M, Walker P, Chapman A, Braun JA. 2016. Reprofiling using zebrafish melanoma model reveals drugs cooperating with targeted therapeutics, Oncotarget., 7,(26), 40348-40361. doi:10.18632/oncotarget.9613
[19] Sorriento D, Laccarino G. 2020. Commentary: Studies in zebrafish demonstrate that CNNM2 and NT5C2 are most likely the causal genes at the blood pressure associated locus on human chromosome 10q24.32, Front. Cardiovasc. Med., 7, (582101). doi:10.3389/fcvm.2020.582101
Downloads
Published
Issue
Section
License
Copyright (c) 2020 Niken Indriyanti (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
