Optimization of Extraction Method of Black Betel Leaf (Piper Sp.) Against Secondary Metabolite Content
DOI:
https://doi.org/10.30872/mpc.v13i.318Keywords:
Black betel (Piper sp.), Extraction method, extract yield, thin layer chromatographic profileAbstract
The black betel plant (Piper Sp.) Has been empirically used by the public for a treatment. This plant has the potential to be developed into pharmaceutical products made from natural ingredients, so an extraction method is needed that can be used as a standard in the development of this plant. The research aims to determine the effect of stirring speed, solid-liquid ratio and sample surface area in the extraction method and to determine the profile of the extraction method. the optimum black betel leaf based on the yield parameters of the extract and the TLC profile. This research is an experimental study conducted by extracting black betel leaves using the maceration method which is influenced by several conditions, namely, with a stirring speed of 300 rpm; 400 rpm; 500 rpm, 1: 5 solid to liquid ratio; 1:10; 1:15 and the surface area of the chopping sample using a blender; 1x1 cm; 2x2 cm. Then the extract yield was calculated and the TLC profile observations were made. The results showed that the stirring speed, the solid-liquid ratio and the surface area of the sample affected the yield of the extract. However, the stirring speed and surface area of the sample did not affect the stain pattern on the TLC profile, while the liquid solid ratio affected the stain pattern on the TLC profile, and the optimum extraction method for the extract yield on black betel leaf was stirring speed of 300 rpm, solid to liquid ratio. ratio of 1:15 and the surface area of the chopped sample using a blender.
References
[1] P. Lestari, ?studi tanaman khas sumatera utara yang berkhasiat obat,? J. Farmanesia, vol. 9, no. 11, pp. 11?21, 2016.
[2] I. Ahmad et al., ?Pharmacognostic and Cytotoxicity Evaluation of Indonesia Native Plant of Piper acre Blume Leaves ( Piperaceae ),? Pharmacogn. J., vol. 9, no. 3, pp. 400?404, 2017.
[3] N. Maulidha, A. Fridayanti, and M. A. Masruhim, ?uji aktivitas antioksidan ekstrak daun sirih hitam (piper sp.) Terhadap dpph (1,1-diphenyl-2-picryl hydrazyl),? J. Sains dan Kesehat., vol. 1, no. 1, pp. 16?20, 2015.
[4] F. S. Siharis and I. Fidrianny, ?ethnopharmacology and activity determination of a plant in moronene ethnic at tobu hukaea-laea bombana-south east sulawesi,? Indones. Nat. Reaserch Pharm. J., vol. 1, no. 1, 2016.
[5] N. I. Z. Junairiah, Ni?matuzahroh, ?Summary for Policymakers,? in Climate Change 2013 - The Physical Science Basis, vol. 3, no. 2, Intergovernmental Panel on Climate Change, Ed. Cambridge: Cambridge University Press, 2018, pp. 1?30.
[6] M. Rahminiwati, Y. Lestari, A. A. Mustika, and A. Zaim, ?Piper and Zingiberace are Potencial as Antibacterial Agent of Chronic Respiratory Disease in Poultry,? Proceeding 3 Jt. Int. Meet., vol. 2, 2014.
[7] M. A. U. Leba, Buku Ajar?: Ekstraksi dan real kromatografi, 1st ed. Yogyakarta: Deepublish, 2017.
[8] K. H. Dewi, D. Silsia, L. Susanti, and M. Markom, ?Ekstraksi Teripang Pasir ( Holothuria Scabra ) Sebagai Sumber Testosteron Pada Berbagai Kecepatan dan Lama Pengadukan,? Pros. Semin. Nas. Tek. Kim., no. 2009, pp. 1?7, 2010.
[9] A. T. Budiyati, Eni, ?Pengaruh Kecepatan Putaran Pengaduk Terhadap Konsentrasi Polifenol, Kca, Dan De Pada Ekstraksi Polifenol Dari Kulit Apel Malang,? Simp. Nas. RAPI XII, no. ISSN 1421-9612, pp. 82?88, 2013.
[10] G. Rifai, I. W. Rai Widarta, and K. Ayu Nocianitri, ?Pengaruh Jenis Pelarut Dan Rasio Bahan Dengan Pelarut Terhadap Kandungan Senyawa Fenolik Dan Aktivitas Antioksidan Ekstrak Biji Alpukat (Persea Americana Mill.),? J. Ilmu dan Teknol. Pangan, vol. 7, no. 2, p. 22, 2018.
[11] A. Noviyanty and C. A. Salingkat, ?The Effect of Solvent Ratio to the Quality of Extracts from the Red Dragon Fruit Peel ( Hylocereus polyrhizus ),? KOVALEN, 5(3) 280-289, vol. 5, no. 3, pp. 280?289, 2019.
[12] N. P. Noviantari, L. Suhendra, and N. M. Wartini, ?Pengaruh Ukuran Partikel Bubuk dan Konsentrasi Pelarut Aseton Terhadap Karakteristik Ekstrak Warna Sargassum Polycystum,? J. Rekayasa dan Manaj. Agroindustri, vol. 5, no. 3, pp. 102?112, 2017.
[13] R. Maulida and A. Guntarti, ?pengaruh ukuran partikel beras hitam (oryza sativa l.) Terhadap rendemen ekstrak dan kandungan total antosianin,? Pharmaciana, vol. 5, no. 1, pp. 9?16, 2015.
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Copyright (c) 2021 Jamal Habibullah, Risna Agustina, Fajar Prasetya (Author)
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